Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

Intestinal Epithelial Cells Ilium

Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

Lung Small Airway Epithelial Cells UMIX

25NOVAPOOL™ 10-Donor Mixed-Gender Pooled Human Hepatocytes

NhSAE-P0: Normal Human Small Airway Epithelial Cells UMIX

Isolated from the fifth generation of bronchi down to small airways near 1mm in diameter.  The cell composition consists of undifferentiated ciliated, non-ciliated, goblet, and basal cells.

Proliferating NhSAE-P0 cells have been characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), and tight junctions (Zonula Occludens-1).

The NhSAE-P0 cells are guaranteed to have minimum population doublings of at least 20 (from P0 to P2) following the instructions and conditions provided by Novabiosis.

Cells per vial: 500,000

Product Shipping & Storage

Shipping Conditions: Dry Ice or LN2
Storage Conditions: LN2 Storage

Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage Dewar. Cells are viable for at least 2 years when stored under these conditions.

Quality Control Testing

QC testing results provided will consist of the following:

  • Attachment from P0 thaw: Typically 20-30% of initial count
  • Number of population doublings: Typically 25 (P0 to P2 in BEGM)
  • Average population doubling time: Typically 40 ± 5 hours (P1 to P2 cells)
  • Transepithelial resistance at 21 days ALI: Typically range from 500 to 2000 Ω (P0 cells starting material, 12mm)
  • Percent ciliated cells at 21 days ALI: Typically >50% at P1 (P0 cells starting material)
  • Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
  • Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2. Potentially HLTV-1 and HLTV-2 (if performed).
  • Culture results include (positive or negative): Gram +, Gram -, Mycoplasm and Fungi

Product Warranty

CULTURES HAVE A LIMITED LIFESPAN IN VITRO.

Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.

Cryopreserved proliferating NhSAE-p0 are assured to be viable and functional when thawed and maintained properly.

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THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.

WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are  tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.

Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.