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NhGEC: Normal Human Glomerular Endothelial Cells
Specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type.
The NhGEC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhGEC are isolated by CD31 positive selection as a pure population.
The NhGEC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.
The NhGEC will be evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by immunofluorescence.
NhGEC can be expanded between 5 to 8 passages, while maintaining their particular characteristics. NhGEC are terminally differentiated cells and present no significant differentiation capacity.
Cells per vial: 500,000
Shipping Conditions: Dry Ice or LN2
Storage Conditions: LN2 Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage Dewar. Cells are viable for at least 2 years when stored under these conditions.
QC testing results provided will consist of the following:
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhGEC are assured to be viable and functional when thawed and maintained properly.
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THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
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