Normal Human Glomerular Endothelial Cells (NhGEC)
$495.00
Normal Human Glomerular Endothelial Cells (NhGEC) are specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type.
The NhGEC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhGEC are isolated by CD31 positive selection as a pure population.
The NhGEC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.
The NhGEC will be evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by immunofluorescence.
NhGEC can be expanded between 5 to 8 passages, while maintaining their particular characteristics.
NhGEC are terminally differentiated cells and present no significant differentiation capacity.
The NhGEC are for research use only and not approved for human use, or for in vitro diagnostic testing.
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Human Kidney Glomerular Endothelial Cells Product Literature
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Product Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Quality Control Testing
QC testing results provided will consist of the following:
Date of manufacture: MM/DD/YYYY
Attachment efficiency post thaw: Reported
Number of population doublings: 5-8
Immunoflouresence: CD31, vWF | Ve-Cadherin
Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, and HLTV-1 and HLTV-2 (only if performed)
Culture results include (positive or negative): Mycoplasma, fungi and yeast
Product Warranty
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhGEC are assured to be viable and functional when thawed and maintained properly.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
