Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

Intestinal Epithelial Cells Ilium

Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

Lung Small Airway Epithelial Cells – Passage 1

25NOVAPOOL™ 10-Donor Mixed-Gender Pooled Human Hepatocytes

NhSAE-P1: Normal Human Small Airway Epithelial Cells homogenous basal cell population at passage 1

Initially isolated as NhSAE-P0 cells from the fifth generation of bronchi down to small airways near 1mm in diameter. The NhSAE-P0 cells are then expanded on plastic using BEGM, CRC or Dual Smad expansion media to isolate out an homogenous basal cell population known as NhSAE-P1.

Proliferating NhSAE-P1 cells have been characterized by morphological assessment by light microscopy. The NhSAE-P1 homogenous basal cell population was then further QC tested after differentiating the NhSAE-P1 cells into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).

The NhSAE-P1 cells are guaranteed to have minimum population doublings of at least 20 (from P1 to P3) following the instructions and conditions provided by Novabiosis.

Cells per vial: 500,000

Product Shipping & Storage

Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.

Quality Control Testing

QC testing results provided will consist of the following:

  • Attachment from P1 thaw: Typically 50-75% of initial count
  • Number of population doublings: Typically 20 (P1 to P3 in your preferred BEGM conditions)
  • Average population doubling time: Typically 40 ± 5 hours (P1 to P2 cells)
  • Transepithelial resistance at 21 days ALI: Typically range from 500 to 2000 Ω (P1 cells starting material, 12mm)
  • Percent ciliated cells at 21 days ALI: Typically >50% of P1 cells


  • Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
  • Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, potentially HLTV-1 and HLTV-2 ( if performed)
  • Culture results include (positive or negative): Gram +, Gram -, Mycoplasm and Fungi

Product Warranty


Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.

Cryopreserved proliferating NhSAE-P1 are assured to be viable and functional when thawed and maintained properly.

THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.

WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are  tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus.  In some cases, but not all, HTLV-1 and 2 are tested and reported.

Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.