Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Tracheal Epithelial Cells- P0 (NhTE-P1) are isolated as NhTE-P0 cells from the trachea of healthy human donors but do not contain epithelial cells from the primary bronchus. Each vial of the Proliferating NhTE-P1 cells were expanded on plastic using BEGM, CRC or Dual Smad expansion media to isolate out an homogenous basal cell population.
Proliferating NhTE-P1 cells have been characterized by morphological assessment by light microscopy. The NhTE-P1 homogenous basal cell population was then further QC tested after differentiating the NhTE-P1 cells into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5). The NhTE-P1 cells are guaranteed to have a minimum of at least 20 population doublings from P1 to P3 following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.