Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage dewar. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Bronchial Epithelial Cells- P0 (NhBE-P0) are isolated from the bronchus at the bifurcation point to the fourth generation of bronchi. The cell composition consists of undifferentiated ciliated, non-ciliated, goblet, and basal cells.
Proliferating NhBE-P0 cells have been characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhBE-P0 cells are guaranteed to have minimum population doublings of at least 20 (from P0 to P2) following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.