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NhKP: Normal Human Kidney Podocytes
Terminally differentiated cells found lining the outer surface of the glomerular capillaries.
The NhKP are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhKP are isolated by nephrin and podocin positive selection as a pure population, then cryopreserved.
The podocytes have foot processes, that form the slit diaphragm to allow ultrafiltration of the blood based on size and charge and allows the filtration of…, electrolytes, and small and midsized solutes while restricting the passage of anionic molecules and macromolecules.
Expression of slit diaphragm molecules nephrin and podocin will be tested by immunostaining and qPCR. Absence of CD-31 and PDGFβ-R (endothelia and mesangial markers, respectively) will be assessed by immunofluorescence and qPCR.
Podocytes cannot be cultured for more than 1 or 2 passage without loss of their particular traits (like foot processes and expression of slit diaphragm proteins).
Cells per vial: 50k-99k | 100k-200k | 250k-400k
3 additional vials/ampules will be shipped per cell vial:
Shipping Conditions: Dry Ice or LN2
Storage Conditions: LN2 Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage Dewar. Cells are viable for at least 2 years when stored under these conditions.
QC testing results provided will consist of the following:
Date of Manufacture: MM/DD/YYYY
Immunostaining and qPCR: Nephrin, Podocin,
Absence of CD-31 and PDGFβ-R
Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, and HLTV-1 and HLTV-2 (only if performed)
Culture results include (positive or negative): Mycoplasma, fungi and yeast
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhKP cells are assured to be viable and functional when thawed and maintained properly.
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THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
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