Kidney Mesangial Cells

Kidney Mesangial Cells

Kidney Mesangial Cells

Kidney Mesangial Cells

Intestinal Epithelial Cells Ilium

Kidney Mesangial Cells

Kidney Mesangial Cells

Kidney Mesangial Cells

Kidney Mesangial Cells

25NOVAPOOL™ 10-Donor Mixed-Gender Pooled Human Hepatocytes

NhKM:  Normal Human Kidney Mesangial Cells

Found within the glomerulus and are derived from the cortex of the kidney during the isolation process. These cells “fill the gap” between adjacent capillary loops surrounded by the glomerular basement membrane (GBM) and the podocytes. The primary function of NhKM is to remove trapped residues and aggregated protein from the basement membrane.  In addition, they aid filtration by constituting part of the glomerular capillary tuft structure that filters fluids to produce urine.

The NhKM cells are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhKM cells are isolated by PDGF-R beta positive selection as a pure population, then cryopreserved.

Purity is analyzed using immunofluorescence microscopy for PDGFRβ, vimentin, and α smooth muscle actin. Cells are terminally differentiated and can be expanded for at least 15 population doublings. Mesangial cells have an intermediate phenotype, sharing features of fibroblasts and smooth muscle cells.

When grown in one of the common media formulations for eukaryotic cells (e.g. MEM, DMEM, Waymouth, RPMI 1640), these cells retain their in vivo phenotype as stellate, arborized elements in monolayer culture. Homogeneous cultures of Mesangial cells resemble smooth muscle cells or fibroblasts. On average, 15–20 passages in culture can been obtained from NhKM cells.

The NhKM cells are terminally differentiated.

Cells per vial: 500,000

 

Additional information

Donor

001 (500,000 per/vial), 001a (500,000 cells/vial), 001c (500,000 cells/vial), 002 (500,000 per/vial), YIA-M1 (500,000 cells per/vial), 004a (500,000 cells per/vial)

Product Shipping & Storage

Shipping Conditions: Dry Ice or LN2
Storage Conditions: LN2 Storage

Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage Dewar. Cells are viable for at least 2 years when stored under these conditions.

Quality Control Testing

QC testing results provided will consist of the following:

  • Immunostaining and a PCR: At a minimum desmin, vimentin, and smooth muscle myosin and actin (SMA).
  • Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
  • Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, and HLTV-1 and HLTV-2 (only if performed)
  • Culture results include (positive or negative): Mycoplasma, fungi and yeast

Product Warranty

CULTURES HAVE A LIMITED LIFESPAN IN VITRO.

Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.

Cryopreserved proliferating NhKM cells are assured to be viable and functional when thawed and maintained properly.

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THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.

WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are  tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus.  In some cases, but not all, HTLV-1 and 2 are tested and reported.

Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.