Normal Human Bronchial Epithelial Cells-P1 (NhBE-P1)
$595.00
Proliferating Normal Human Bronchial Epithelial Cells- P1 (NhBE-P1) are isolated, initially as NhBE-P0, from the bronchus at the bifurcation point to the fourth generation of bronchi. The original cell composition consisted of a mix of differentiated ciliated, non-ciliated, goblet, and basal cells. After culturing the mixed NhBE-P0 in BEGM, CRC or Dual Smad culture conditions, the result is a homogenous population of basal cells, or NhBE-P1.
Proliferating NhBE-P1 cells have been characterized by morphological assessment by light microscopy. The NhBE-P1 homogenous basal cell population was then further QC tested after differentiating the NhBE-P1 cells (using Novabiosis ALI media) Proliferating Normal Human Bronchial Epithelial Cells-P1 (NhBE-P1) into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhBE-P1 cells are guaranteed to have minimum population doublings of at least 20 (from P1 to P3) following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
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Cryopreserved Human Bronchial Epithelial P-1 Product Literature
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Product Storage
Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage dewar. Cells are viable for at least 2 years when stored under these conditions.
Quality Control Testing
QC testing results provided will consist of the following:
Date of manufacture: MM/DD/YYYY
Attachment from P1 thaw: Typically 75% of initial count
Number of population doublings: Typically 20 (P1 to P3 in BEGM)
Average population doubling time: Typically 40 ± 5 hours (P1 to P2 cells)
Transepithelial resistance at 21 days ALI: Typically range from 500 to 2000 Ω
Percent ciliated cells at 21 days ALI: Typically >50% of P1 cells
Donor Characteristics Include: Donor age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities
Serology results include (positive or negative): CMV, EBV HBV, HCV, HIV-1, HIV-2, and HLTV-1 and HLTV-2 (only if performed)
Culture results include (positive or negative): Gram +, Gram -, Mycoplasm and Fungi
Product Warranty
CULTURES HAVE A LIMITED LIFESPAN IN VITRO.
Novabiosis guarantees the performance of its cells only if following Novabiosis specific use instructions exclusively, and the recommended protocols are followed. The performance of the cells are not guaranteed if any modifications are made.
Cryopreserved proliferating NhTE-p0 are assured to be viable and functional when thawed and maintained properly.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
WARNING: Primary human cell products are derived from whole human organs, thus should be treated as potentially infectious. Blood samples from the donor are tested and found non-reactive using FDA approved test kits in CLIA certified labs and are testing for the presence of HIV-I/II, Hepatitis B Virus and Hepatitis C Virus. In some cases, but not all, HTLV-1 and 2 are tested and reported.
Test results do not offer complete assurance that HIV-I/II, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the appropriate Biological Safety Level in order to minimize exposure of potentially infectious products.
